Crivelli, A.J. and Quatre, C. (2001) La gambusie Gambusia affinis holbrooki Girard, 1859., pp. 282-283
| Species | Development state | Trait | Primary Data | Secondary Data |
| Gambusia affinis | Spawning conditions | Mating system | Male very aggresive, wants to deposit their sperm in the biggest number of females into a special pocket [Sperm can remain viable for three months] | No category |
| Gambusia affinis | Spawning conditions | Spawning season | Beginning of June until end of September | ['June', 'September'] |
| Gambusia affinis | Spawning conditions | Spawning release | Females can have up to six groups of frys during a season | No category |