Koya, Y. and Itazu, T. and Inoue, M. (1998) Annual reproductive cycle based on histological changes in the ovary of the female mosquitofish, Gambusia affinis, in central Japan. Ichthyological Research [Ichthyol. Res.]., pp. 241-248
| Species | Development state | Trait | Primary Data | Secondary Data |
| Gambusia affinis | Female | Onset of oogenesis | April | ['April'] |
| Gambusia affinis | Female | Intensifying oogenesis activity | May-June | ['May', 'June'] |
| Gambusia affinis | Female | Maximum GSI value | 23-24.1 [From late June to early October] | 23.55 percent |
| Gambusia affinis | Female | Resting period | 1.0-1.1 [From January to April] | 1.05 months |
| Gambusia affinis | Spawning conditions | Spawning temperature | 17-21 | 19.0 °C |
| Gambusia affinis | Spawning conditions | Spawning release | Viviparous | No category |