Koya, Y. and Inoue, M. and Naruse, T and Sawaguchi, S. (2000) Dynamics of oocyte and embryonic development during ovarian cycle of the viviparous mosquitofish Gambusia affinis. Fisheries Science, pp. 63-70
| Species | Development state | Trait | Primary Data | Secondary Data |
| Gambusia affinis | Egg | Oocyte diameter | Mature oocytes were seen in follicles greater than 1.6 mm in diameter, and the number increased as the diameter became larger | 1.6 mm |
| Gambusia affinis | Egg | Egg size after water-hardening | Fertilised eggs began to appear in 1.8-1.9 mm follicles (2.4%) adn Increased to 16.0% in 1.90-2.0 mm follicles [The fertilized eggs were sligtly larger in diameter than mature oocytes] | 1.85 mm |
| Gambusia affinis | Egg | Incubation time | The ovarian events between two successive parturitions were summarized as follows: active vitellogenesis (days 0-3), maturation and fertilization (days 3-5), progress of embryonic developmentand beginning of vitellogenesis for the following gestation (day 10), and pre-parturition and progress of vitellogenesis (day 20). | 1.5 days |
| Gambusia affinis | Egg | Temperature for incubation | 25°C | 25.0 °C |
| Gambusia affinis | Egg | Degree-days for incubation | 100-120 [Seems to 5-6 days at 25°C] | 110.0 °C * day |
| Gambusia affinis | Female | Maximum GSI value | 20-22% , every 22 days during the reproductive season | 21.0 percent |
| Gambusia affinis | Spawning conditions | Spawning temperature | Mosquitofish need more than 18°C temperature and 12.5 h daylength for their reproduction, and that brood interval is kept almost constant if the water temperature and photoperiod are constant. | 18.0 °C |