Koya, Y. and Iwase, A. (2004) Annual reproductive cycle and rate of the spermatogenic process in male mosquitofish Gambusia affinis. Ichthyol. Res., pp. 131-136
| Species | Development state | Trait | Primary Data | Secondary Data |
| Gambusia affinis | Female | Oocyte development | Viviparous | No category |
| Gambusia affinis | Male | Onset of spermatogenesis | Early May | ['May'] |
| Gambusia affinis | Male | Main spermatogenesis activity | Mid-May to early June [Increase from 0.9-3.2%] | ['May', 'June'] |
| Gambusia affinis | Male | Maximum GSI value | 3.8 [June to July] | 3.8 percent |
| Gambusia affinis | Male | Resting period | Resting period is October to April [The GSI values (0.9-2.1%) from January to May] | 1.5 months |