Hughes, L.A. (1985) Seasonal changes in fecundity and size at first reproduction in an Indiana population of the mosquitofish Gambusia affinis. American Midland Naturalist, pp. 30-36
| Species | Development state | Trait | Primary Data | Secondary Data |
| Gambusia affinis | Female | Length at sexual maturity | Adult females > 2.3 cm | 2.3 cm |
| Gambusia affinis | Spawning conditions | Spawning season | In both years, no females with embryos were found before April. High percentages of adult females had embryos in June and july and (in 1983) in early august. By late september 1982 no females with embryos were found; and in 1983 only a very few gravid females were found in late September | ['April', 'June', 'September'] |