Ankley, G.T., Jensen, K.M., Kahl, M.D., Korte, J.J. and Makynen, E.A., 2001. Description and evaluation of a short‐term reproduction test with the fathead minnow (Pimephales promelas). Environmental Toxicology and Chemistry: An International Journal, 20(6), pp.1276-1290.
| Species | Development state | Trait | Primary Data | Secondary Data |
| Pimephales promelas | Egg | Degree-days for incubation | 25; 4-5 | 112.5 °C * day |
| Pimephales promelas | Larvae | Full yolk-sac resorption | takes around 4 days at 25 C | 4.0 °C * day |