Piller, K.R. and Burr, B.M., 1999. Reproductive biology and spawning habitat supplementation of the relict darter, Etheostoma chienense, a federally endangered species. Environmental Biology of Fishes, 55(1), pp.145-155.
| Species | Development state | Trait | Primary Data | Secondary Data |
| Etheostoma chienense | Egg | Egg size after water-hardening | 1.70-2.00 | 1.85 mm |